Program

histo

Friday 16th February 2018: Macroscopic Analysis and Sampling for Quantitative Microscopy

2 pm	Get-together and Welcome in the Department of Anatomy
2.30 pm	Introdutory Remarks:"Priciples of Processing and Sampling of Human Placentas for Quantitative Microscopy including 3D Microscopy" (H.-G. Frank), Q and A
3 pm	- Practical demonstration of entry routine, macroscopic data acquisition, and approach to systematic random sampling of placentas in the laboratories of the department, Q and A. - Practical demonstration of sampling for quantitative 3D Microscopy - Participants will have ample opportunity to train sampling of peripheral villous trees under guidance of experienced staff members of the department. - Participants will have the opportunity to isolate peripheral villous trees from previously deparaffinated archived tissues. - Fixation protocols (for freshly isolated villous trees), dehydration, and mounting of isolated villous trees will be demonstrated and mounting of pre-processed villous trees can be trained by the participants.
7 pm	Q and A session and discussion of staining protocols and immunohistochemical protocols for isolated peripheral villous trees (H.-G. Frank)
8 pm	Lunch at a restaurant in walking distance from the department

9 am	“The camera lucida principle and its application to the biological trees, especially the villous tree” (H.-G. Frank)
9.30 am	“An Overview on the Practical Process of 3D Microscopic Analysis of Isolated Villous Trees” (E. Haeussner)
10 am	- Step for Step demonstration of a routine evaluation under the microscope (E. Haeussner) - Training and exercise at microscopes by the participants, supervised by experienced staff - Q and A
11.30 am	Lunch
12.30 pm	“Data Structures obtained by 3D Microscopic Analysis and their Evaluation” (E. Haeussner)
1 pm	- Step by Step demonstration of an advanced evaluation including placement of markers during analysis - Training and exercise by the participants supervised by experienced staff - Demonstration of examples with immunohistochemical protocols involved in sample preparation. - Q and A, discussion of questions of participants with regard to their own running or planned projects
ca. 4.30 pm	End